The complex of plant nematodes, which associated with Scots pine seedlings in Ukrainian forestry nurseries, was investigated. We have detected 67 species of plant nematodes, belonging to 61 genera, 31 families and 7 orders. Representatives of orders Tylenchida and Rhabditida were the most typical. Species of Ditylenchus dipsaci (frequency of occurrence is 52,3 %), Aglenchus agricola (72,6 %), Aphelenchoides asterocaudatus (58,3 %), Acrobeloides buetschlii (95,4 %) were dominating. According to the ecotrophic characteristic, all species are divided into 4 groups: phytohelminths (17 species), mycohelminths (9 species), saprobionts (36 species) and predators (5 species). Number of nematodes was lower in the beginning of vegetative period and considerably increased to the end. Deterioration of health status of plants directly depends on the intensity of accumulation plant parasites in plant rhizosphere. Close indicators of acidity and mechanical structure of soil testifies to absence of their considerable influence on number and a species diversity of plant nematodes.